Pre-concentration of non-uniform field electrophoresis for sample introduction of capillary electrophoresis.
J Chromatogr A
; 932(1-2): 13-20, 2001 Oct 12.
Article
em En
| MEDLINE
| ID: mdl-11695858
ABSTRACT
A new sample introduction method of capillary electrophoresis, in which field-amplified sample injection was combined with a pre-concentration of non-uniform field electrophoresis, is presented in this paper. With an additional pre-concentration voltage applied to sample solution, a non-uniform electric field was generated, with which analytical cations or anions were pre-concentrated around an electrode adjacent to the injection end of capillary. After the pre-concentration, analytical ions were injected into the capillary and stacked at the boundary between sample and buffer solution inside capillary by field-amplified injection technique. In contrast to the conventional field-amplified injection, larger concentration factor and higher analytical sensitivity were obtained with the improved pre-concentration method. Its concentration factor was about 10 approximately 15 fold as that of field-amplified sample injection.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Eletroforese Capilar
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article