Plasmalogen degradation by oxidative stress: production and disappearance of specific fatty aldehydes and fatty alpha-hydroxyaldehydes.
Free Radic Biol Med
; 31(10): 1263-71, 2001 Nov 15.
Article
em En
| MEDLINE
| ID: mdl-11705705
ABSTRACT
Plasmalogens are often considered as antioxidant molecules that protect cells from oxidative stress. Their vinyl ether bond could indeed be among the first targets for newly formed radicals. However, the long chain aldehydes released from plasmalogens were seldom studied and possible injurious or harmless effects were poorly examined. Thus, the sensitivity of the vinyl ether bond of plasmalogens was investigated in a cerebral cortex homogenate under UV irradiation- or Fe2+/ascorbate-induced peroxidation. Kinetics of aldehyde production was followed by gas chromatography/mass spectrometry. This confirmed that plasmalogens were highly sensitive to oxidative stress (70% cleavage after 90 min UV irradiation and 30% after 30 min of Fe2+/ascorbate). The aldehydes corresponding to sn-1 position 160, 180, or 181 were poorly detected. Conversely, oxidation of plasmalogens yielded preferentially 150, 170, and 171 aldehydes under UV and the alpha-hydroxyaldehydes 160-OH and 180-OH following a Fe2+/ascorbate oxidation. Kinetics showed that free aldehydes and above all free alpha-hydroxyaldehydes disappeared from the medium as soon as produced. Consequently, the behavior of these released aldehydes in the tissues has to be investigated in order to ascertain the protective effect of plasmalogens against oxidation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmalogênios
/
Córtex Cerebral
/
Estresse Oxidativo
/
Aldeídos
/
Ácidos Graxos
Limite:
Animals
Idioma:
En
Ano de publicação:
2001
Tipo de documento:
Article