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TSC2 missense mutations inhibit tuberin phosphorylation and prevent formation of the tuberin-hamartin complex.
Nellist, M; Verhaaf, B; Goedbloed, M A; Reuser, A J; van den Ouweland, A M; Halley, D J.
Afiliação
  • Nellist M; Department of Clinical Genetics, Erasmus University, 3015 GE Rotterdam, The Netherlands. nellist@kgen.fgg.eur.nl
Hum Mol Genet ; 10(25): 2889-98, 2001 Dec 01.
Article em En | MEDLINE | ID: mdl-11741832
ABSTRACT
Tuberous sclerosis (TSC) is an autosomal dominant disorder characterized by a broad phenotypic spectrum that includes seizures, mental retardation, renal dysfunction and dermatological abnormalities. Inactivating mutations to either of the TSC1 and TSC2 tumour suppressor genes are responsible for the disease. TSC1 and TSC2 encode two large novel proteins called hamartin and tuberin, respectively. Hamartin and tuberin interact directly with each other and it has been reported that tuberin may act as a chaperone, preventing hamartin self-aggregation and maintaining the tuberin-hamartin complex in a soluble form. In this study, the ability of tuberin to act as a chaperone for hamartin was used to investigate the tuberin-hamartin interaction in more detail. A domain within tuberin necessary for the chaperone function was identified, and the effects of TSC2 missense mutations on the tuberin-hamartin interaction were investigated to allow specific residues within the central domain of tuberin that are important for the interaction with hamartin to be pin-pointed. In addition, the results confirm that phosphorylation may play an important role in the formation of the tuberin-hamartin complex. Although mutations that prevent tuberin tyrosine phosphorylation also inhibit tuberin-hamartin binding and the chaperone function, our results indicate that only hamartin is phosphorylated in the tuberin-hamartin complex.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Esclerose Tuberosa / Proteínas / Mutação de Sentido Incorreto Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2001 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas Repressoras / Esclerose Tuberosa / Proteínas / Mutação de Sentido Incorreto Tipo de estudo: Prognostic_studies Limite: Animals / Humans Idioma: En Ano de publicação: 2001 Tipo de documento: Article