Enhanced animal growth via ligand-regulated GHRH myogenic-injectable vectors.
FASEB J
; 16(3): 426-8, 2002 Mar.
Article
em En
| MEDLINE
| ID: mdl-11790726
ABSTRACT
Regulated animal growth occurred following a single electroporated injection of a mixture of two plasmids (10 microg of DNA), one expressing the GeneSwitch regulator protein, the other an inducible growth hormone releasing hormone (GHRH) gene, into the tibialis anterior muscles of adult SCID mice. Administration of the ligand mifepristone (MFP) up-regulated GHRH expression, as shown by elevations of IGF-I levels, and when MFP dosing was withdrawn, IGF-I returned to baseline levels. Five cycles of IGF-I induction were observed during a five-month period. Chronic MFP dosing for 25 days increased lean body mass, weight gain, and bone mineral density significantly compared with non-MFP treated controls. In summary, long-term drug-regulated GHRH expression was achieved following plasmid-based gene therapy, and chronic induction of GHRH expression in adult animals led to improvements in weight gain and body composition.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aumento de Peso
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Mifepristona
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Hormônio Liberador de Hormônio do Crescimento
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Músculo Esquelético
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Vetores Genéticos
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article