Your browser doesn't support javascript.
loading
Maintenance and characterization of an Epstein Barr virus-infected CD56-negative T cell lymphoma.
Maekawa, Izuru; Satoh, Hitoshi; Aoki, Naoto; Morishita, Yasuyuki; Tsukamoto, Norifumi; Karasawa, Masamitsu; Nonaka, Yasunobu; Shiota, Mami; Nojima, Yoshihisa; Mori, Shigeo.
Afiliação
  • Maekawa I; Division of Pathology, Institute of Medical Science, University of Tokyo, Minato-ku, Tokyo 108-8639, Japan. izm9182@ims.u-tokyo.ac.jp
Jpn J Cancer Res ; 93(1): 61-9, 2002 Jan.
Article em En | MEDLINE | ID: mdl-11802809
T cell lymphoma carrying Epstein Barr virus (EBV(+) TL) is very rare among Western countries while it is much more common among Japanese. Here we report an EBV(+) TL which has been maintained for years by the use of mice with severe combined immune deficiency (SCID) mice. Lymphoma was obtained from a 55-year-old male suffering from oculomotor nerve palsy and lymphadenopathy. A small piece of biopsied tumor was transplanted into SCID mice and the lymphoma has been maintained for over 3 years with passages every 2 - 3 weeks. The maintained lymphoma, termed as TMS24, and the original lymphoma cells showed identical phenotype and genotype, including diffuse medium-sized cell morphology lacking granules, suppressor / cytotoxic immunophenotype and identical T cell receptor beta-chain gene rearrangement mode. Further, both were shown to carry an identical EBV clone in terms of the number of terminal repeats and the latency II-type restricted gene expression profile. Cytogenetically, TMS24 retained two characteristic chromosomal translocations of t(1;18)(q32;q21) and t(6;12)(p21;q24). Since only one cell line with such characters has been reported previously, TMS24 should be useful for detailed analysis of EBV(+) TL.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem Celular / Linfoma de Células T / Células Clonais / Herpesvirus Humano 4 / Antígeno CD56 Limite: Animals / Humans / Male / Middle aged Idioma: En Ano de publicação: 2002 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Linhagem Celular / Linfoma de Células T / Células Clonais / Herpesvirus Humano 4 / Antígeno CD56 Limite: Animals / Humans / Male / Middle aged Idioma: En Ano de publicação: 2002 Tipo de documento: Article