Changes in hepatic vitellogenin mRNA levels during oocyte development in the Japanese eel, Anguilla japonica.
Gen Comp Endocrinol
; 125(1): 9-16, 2002 Jan.
Article
em En
| MEDLINE
| ID: mdl-11825029
ABSTRACT
The induction of vitellogenesis is a complex process requiring coordinated control and expression of many hepatic gene products, such as vitellogenin (VTG). To investigate the regulation of VTG synthesis, knowledge of the molecular genetics of VTG is required. Here, the authors have isolated a partial cDNA encoding Japanese eel VTG using an immunoscreening technique. This cDNA contained an open reading frame of 1629 bp, predicted to encode 543 amino acid residues, the sequence of which showed high homology with the VTG of other fishes. Northern blot analysis yielded a VTG transcript of approximately 5.8 kb from eel hepatic tissue. Experimentally, VTG synthesis could be induced by treatment with salmon pituitary homogenate. The levels of VTG mRNA in the liver during the artificial maturation of female Japanese eels correspond well to levels of E2 and VTG in the serum.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
RNA Mensageiro
/
Vitelogeninas
/
Anguilla
/
Fígado
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article