Silence of chromosomal amplifications in colon cancer.
Cancer Res
; 62(4): 1134-8, 2002 Feb 15.
Article
em En
| MEDLINE
| ID: mdl-11861394
ABSTRACT
Oncogene activation by gene amplification is a major pathogenetic mechanism in human cancer. Using comparative genomic hybridization, we determined that metastatic human colon cancers commonly acquire numerous extra copies of chromosome arms 7p, 8q, 13q, and 20q. We then examined the consequence of these amplifications on gene expression using DNA microarrays. Of 55,000 transcripts profiled, 2,146 were determined to map to one of the four common colon cancer amplicons and to also be expressed in normal or malignant colon tissues. Of these, only 81 transcripts (3.8%) demonstrated a 2-fold increase over normal expression among cancers bearing the corresponding chromosomal amplification. Chromosomal amplifications are common in colon cancer metastasis, but increased expression of genes within these amplicons is rare.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Amplificação de Genes
/
Neoplasias do Colo
Limite:
Humans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article