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Four chimpanzee monoclonal antibodies isolated by phage display neutralize hepatitis a virus.
Schofield, D J; Satterfield, W; Emerson, S U; Purcell, R H.
Afiliação
  • Schofield DJ; Hepatitis Viruses Section, Laboratory of Infectious Diseases, NIAID, NIH, Bethesda, Maryland 20892, USA. DSCHOFIELD@NIAID.NIH.GOV
Virology ; 292(1): 127-36, 2002 Jan 05.
Article em En | MEDLINE | ID: mdl-11878915
Chimpanzee immunoglobulins are virtually identical to human immunoglobulins and may have clinically useful applications. Four chimpanzee monoclonal antibodies (MAbs) to the hepatitis A virus (HAV) capsid were isolated from a combinatorial cDNA library of gamma1/kappa antibody genes using phage display. Competition assays indicated that three of the MAbs recognized the same or overlapping epitopes, whereas the fourth recognized a different, nonoverlapping epitope on the HAV capsid. All four MAbs neutralized the homologous HAV strain, HM-175, in a radioimmunofocus assay and two of the four MAbs neutralized a heterologous simian HAV strain, AGM-27. From these data, we conclude that the MAbs must recognize at least three epitopes on the HAV capsid. Furthermore, competition assays performed with neutralizing murine MAbs suggested that three of the chimpanzee MAbs recognized epitopes on the HAV capsid which have not been defined previously.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Anti-Hepatite / Pan troglodytes / Vírus da Hepatite A / Anticorpos Monoclonais / Especificidade de Anticorpos Limite: Animals Idioma: En Ano de publicação: 2002 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Anticorpos Anti-Hepatite / Pan troglodytes / Vírus da Hepatite A / Anticorpos Monoclonais / Especificidade de Anticorpos Limite: Animals Idioma: En Ano de publicação: 2002 Tipo de documento: Article