Isolation and characterization of a motile hydrogenotrophic methanogen from rice paddy field soil in Japan.
FEMS Microbiol Lett
; 208(2): 239-43, 2002 Mar 05.
Article
em En
| MEDLINE
| ID: mdl-11959443
ABSTRACT
A hydrogenotrophic motile methanogen was isolated from flooded Japanese paddy field soil. Anaerobic incubation of the paddy soil on H(2)-CO(2) at 20 degrees C led to the enrichment of symmetrically curved motile autofluorescent rods. The methanogenic strain TM20-1 isolated from the culture was halotolerant and utilized H(2)-CO(2), 2-propanol-CO(2), or formate as a sole methanogenic substrate. Based on the 16S rRNA gene sequence similarity (94.8%) with Methanospirillum hungateii, and on the physiological and phenotypic characteristics, TM20-1 was suggested to be a newly identified species belonging to the genus Methanospirillum. This is the first report of isolation of the genus Methanospirillum strain from a rice paddy field.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Methanospirillum
Tipo de estudo:
Prognostic_studies
País/Região como assunto:
Asia
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article