IL-1beta-induced production of metalloproteinases by synovial cells depends on gap junction conductance.
Am J Physiol Cell Physiol
; 282(6): C1254-60, 2002 Jun.
Article
em En
| MEDLINE
| ID: mdl-11997239
ABSTRACT
Synovial cells can form networks connected by gap junctions. The purpose of this study was to obtain evidence for a necessary role of gap junction intercellular communication in protein secretion by synovial cells. We developed a novel assay to measure the enzymatic activity of metalloproteinases (MMPs) produced by synovial cells in response to interleukin-1beta (IL-1beta) and employed the assay to explore the biological function of gap junctions. IL-1beta produced a dose-dependent increase in MMP activity that was blocked by exposure to the gap junction inhibitors 18alpha-glycyrrhetinic acid and octanol for as few as 50 min. The inhibitors produced an immediate and marked reduction in intercellular communication, as assessed by transient current analysis using the nystatin perforated-patch method. These observations suggest that communication through gap junctions early in IL-1beta signal transduction is critical to the process of cytokine-regulated secretion of MMPs by synovial cells.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Membrana Sinovial
/
Metaloendopeptidases
/
Interleucina-1
/
Junções Comunicantes
/
Fibroblastos
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article