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Synapsis of Tn3 recombination sites: unpaired sites destabilize synapses by a partner exchange mechanism.
He, Jiuya; McIlwraith, Michael J; Burke, Mary E; Boocock, Martin R; Stark, W Marshall.
Afiliação
  • He J; Institute of Biomedical and Life Sciences, University of Glasgow, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, UK.
J Mol Biol ; 319(2): 385-93, 2002 May 31.
Article em En | MEDLINE | ID: mdl-12051915
Catalysis of site-specific recombination is preceded by the formation of a synapse comprising two DNA sites and multiple subunits of the recombinase, together with other "accessory" proteins in some cases. We investigated the stability of synapses of Tn3 resolvase-bound res recombination sites, in plasmids containing either two or three res sites. Although synapses are long-lived in plasmids with just two res sites, persisting for tens of minutes, a synapse of any two sites is relatively short-lived in plasmids with three res sites. The three alternative pairwise synapses that can be formed in three-res plasmids re-assort rapidly relative to the rate of recombination. We propose a "partner exchange" mechanism for this re-assortment, involving direct attack on a synapse by an unpaired res site. This mechanism reconciles studies on selective synapsis in multi-res substrates, which imply rapid interchange of synaptic pairings, with studies indicating that synapses of two Tn3res sites are stable.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Recombinação Genética / Elementos de DNA Transponíveis Idioma: En Ano de publicação: 2002 Tipo de documento: Article
Buscar no Google
Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Plasmídeos / Recombinação Genética / Elementos de DNA Transponíveis Idioma: En Ano de publicação: 2002 Tipo de documento: Article