Cyclic guanosine 5' monophosphate (GMP) prevents expression of neuronal nitric oxide synthase and apoptosis in motor neurons deprived of trophic factors in rats.
Neurosci Lett
; 326(3): 201-5, 2002 Jul 05.
Article
em En
| MEDLINE
| ID: mdl-12095656
ABSTRACT
Deprivation of trophic factors induces expression of neuronal nitric oxide synthase (NOS) and nitric oxide production in cultured motor neurons, leading to apoptosis. Motor neuron apoptosis requires the simultaneous production of nitric oxide and superoxide and is associated with increased nitrotyrosine immunoreactivity. Nitric oxide also stimulates cyclic guanosine 5' monophosphate (cGMP) synthesis, which enhances the survival of motor neurons treated with brain derived trophic factor (BDNF). Here we report that cGMP analogs blocked neuronal NOS induction, nitrotyrosine accumulation, and prevented apoptosis for up to 3 day of motor neurons deprived of trophic factors. Low concentrations of exogenous nitric oxide (<100 nM), which are not toxic for BDNF-treated cultures, reversed the protective effect of cGMP. These results suggest that elevation of cGMP could decrease nitric oxide production, and thereby preventing motor neuron apoptosis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Tirosina
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Substâncias de Crescimento
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Receptores de Fator de Crescimento Neural
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GMP Cíclico
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Neurônios Motores
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Óxido Nítrico
Limite:
Animals
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article