A functional screen for Myc-responsive genes reveals serine hydroxymethyltransferase, a major source of the one-carbon unit for cell metabolism.
Mol Cell Biol
; 22(16): 5793-800, 2002 Aug.
Article
em En
| MEDLINE
| ID: mdl-12138190
ABSTRACT
A cDNA library enriched with Myc-responsive cDNAs but depleted of myc cDNAs was used in a functional screen for growth enhancement in c-myc-null cells. A cDNA clone for mitochondrial serine hydroxymethyltransferase (mSHMT) that was capable of partial complementation of the growth defects of c-myc-null cells was identified. Expression analysis and chromatin immunoprecipitation demonstrated that mSHMT is a direct Myc target gene. Furthermore, a separate gene encoding the cytoplasmic isoform of the same enzyme is also a direct target of Myc regulation. SHMT enzymes are the major source of the one-carbon unit required for folate metabolism and for the biosynthesis of nucleotides and amino acids. Our data establish a novel functional link between Myc and the regulation of cellular metabolism.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Glicina Hidroximetiltransferase
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Carbono
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Fenômenos Fisiológicos Celulares
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Proteínas Proto-Oncogênicas c-myc
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article