Affinity Chromatography of Urokinase Using Cefazoline as a Ligand.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 30(6): 635-637, 1998.
Article
em En
| MEDLINE
| ID: mdl-12168002
ABSTRACT
Sepharose 4B-based affinity adsorbent was prepared using cefazoline as a ligand, and epichlorohydrin as an activator. The density of the ligand in the affinity adsorbents was about 43 &mgr;mol/g wet gel. The optimal adsorption pH for urokinase was 6.0, and the optimal adsorption salt concentration was 1.0 mol/L NaCl. The optimal conditions of elution were 0.1 mol/L glycine buffer, pH 9.0, containing 0.5 mol/L NaCl. A crude urokinase with specific activity 500 u/mg protein was purified on an affinity chromatography column (1 cm x 12 cm), yielding a product of specific activity of 49 300 u/mg with 78% recovery and 98.6-fold purification.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1998
Tipo de documento:
Article