[Experimental study on repair of articular cartilage defect in large area with chondrocytes cultured on fascia].
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
; 16(4): 223-7, 2002 Jul.
Article
em Zh
| MEDLINE
| ID: mdl-12181781
OBJECTIVE: To study the biological characteristic and potential of chondrocytes grafting cultured on fascia in repairing large defect of articular cartilage in rabbits. METHODS: Chondrocytes of young rabbits were isolated and sub-cultured on fascia. The large defect of articular cartilage was repaired by grafts of freeze-preserved and fresh chondrocytes cultured on fascia, and free chondrocytes respectively; the biological characteristic and metabolism were evaluated by macroscopic, histological and immunohistochemical observations, autoradiography method and the measurement of nitric oxide content 6, 12, 24 weeks after grafting. RESULTS: The chondrocytes cultured on fascia maintained normal growth feature and metabolism, and there was no damage to chondrocytes after cryopreservation; the repaired cartilage was similar to the normal cartilage in cellular morphology and biological characteristics. CONCLUSION: Chondrocytes could be cultured normally on fascia, which could be used as an ideal carrier of chondrocytes.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cartilagem Articular
/
Condrócitos
/
Fáscia
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2002
Tipo de documento:
Article