Inverse metabolic engineering: a strategy for directed genetic engineering of useful phenotypes.
Biotechnol Bioeng
; 79(5): 568-79, 2002 Sep 05.
Article
em En
| MEDLINE
| ID: mdl-12209828
ABSTRACT
The classical method of metabolic engineering, identifying a rate-determining step in a pathway and alleviating the bottleneck by enzyme overexpression, has motivated much research but has enjoyed only limited practical success. Intervention of other limiting steps, of counter-balancing regulation, and of unknown coupled pathways often confounds this direct approach. Here the concept of inverse metabolic engineering is codified and its application is illustrated with several examples. Inverse metabolic engineering means the elucidation of a metabolic engineering strategy by first, identifying, constructing, or calculating a desired phenotype; second, determining the genetic or the particular environmental factors conferring that phenotype; and third, endowing that phenotype on another strain or organism by directed genetic or environmental manipulation. This paradigm has been successfully applied in several contexts, including elimination of growth factor requirements in mammalian cell culture and increasing the energetic efficiency of microaerobic bacterial respiration.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fenótipo
/
Recombinação Genética
/
Engenharia Genética
/
Metabolismo
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article