A Study of the Myocardial Nuclear Calcium Transport of Rabbit in vitro.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai)
; 29(6): 567-572, 1997.
Article
em En
| MEDLINE
| ID: mdl-12215770
The characteristics of nuclear calcium regulation were investigated in isolated rabbit myocardial nuclei. It was found that calcium concentration in myocardial nuclei was 2.6 fold more than that in myocardial homogenate (P<0.O1), and the nuclear calcium content was only l/6 of the total cellular calcium. Ca-ATPase of myocardial nuclei was [Ca(2+] and [ATP] dependent. [Ca2+] dependent K(a) and V(max) at 2.O mmol/L [ATP] were 226 nmol/L and 3 460 nmol/(h.mg) protein respectively. [ATP] dependent K(m) value and V(max) at 400 nmol/L [Ca2+] were 376.5 &mgr;mol/L and 2 445 nmol/(h.mg) protein respectively. A positive correlation between nuclear 45)Ca2+ transport and Ca-ATPase activity was observed (r=O.945, P<0.01). The above result suggests that myocardial nuclei are able to transport calcium actively. The pathophysiological role of myocardial nuclear calcium transport should be further determined.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Ano de publicação:
1997
Tipo de documento:
Article