Cloning and Sequence Analyzing the Gene of Trichosanthes Trypsin Inhibitor.

ABSTRACT

A DNA probe for Trichosanthes trypsin inhibitor was amplified from the cDNA library of Trichosanthes kirilowii by polymerase chain reaction. Using this probe to screen the cDNA library, a full-length cDNA sequence of the inhibitor was obtained. The open reading frame encodes a Pre-Pro-inhibitor with Pre-, Pro, and mature peptides of 24, 14, and 27 residues, respectively. The deduced amino acid sequence of the mature peptide is consistent with that determined by the primary structure analysis of the protein.