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Microtubule asymmetry during neutrophil polarization and migration.
Eddy, Robert J; Pierini, Lynda M; Maxfield, Frederick R.
Afiliação
  • Eddy RJ; Department of Biochemistry, Weill Medical College of Cornell University, New York, New York 10021, USA.
Mol Biol Cell ; 13(12): 4470-83, 2002 Dec.
Article em En | MEDLINE | ID: mdl-12475966
The development of cell polarity in response to chemoattractant stimulation in human polymorphonuclear neutrophils (PMNs) is characterized by the rapid conversion from round to polarized morphology with a leading lamellipod at the front and a uropod at the rear. During PMN polarization, the microtubule (MT) array undergoes a dramatic reorientation toward the uropod that is maintained during motility and does not require large-scale MT disassembly or cell adhesion to the substratum. MTs are excluded from the leading lamella during polarization and motility, but treatment with a myosin light chain kinase inhibitor (ML-7) or the actin-disrupting drug cytochalasin D causes an expansion of the MT array and penetration of MTs into the lamellipod. Depolymerization of the MT array before stimulation caused 10% of the cells to lose their polarity by extending two opposing lateral lamellipodia. These multipolar cells showed altered localization of a leading lamella-specific marker, talin, and a uropod-specific marker, CD44. In summary, these results indicate that F-actin- and myosin II-dependent forces lead to the development and maintenance of MT asymmetry that may act to reinforce cell polarity during PMN migration.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microtúbulos / Neutrófilos Limite: Humans / Male Idioma: En Ano de publicação: 2002 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Microtúbulos / Neutrófilos Limite: Humans / Male Idioma: En Ano de publicação: 2002 Tipo de documento: Article