Expression of voltage-dependent calcium channels in the embryonic rat midbrain.
Brain Res Dev Brain Res
; 139(2): 189-97, 2002 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-12480133
ABSTRACT
The diversity of expression of high-voltage activated voltage-dependent calcium channels (VDCC) was investigated with whole-cell voltage-clamp recordings from dissociated embryonic rat ventral mesencephalic cells over a 7-day culture period. Cell phenotype was identified post-recording by fluorescent immunocytochemistry as tyrosine hydroxylase positive (TH+) or glutamic acid decarboxylase positive (GAD+). Both TH+ and GAD+ cells displayed high-threshold calcium (Ca(2+)) currents activated by depolarisations positive to -60 mV. In both cell types, pharmacological dissection using selective VDCC inhibitors, omega-agatoxin IVA (Aga IVA), omega-conotoxin GVIA (GVIA) and nifedipine demonstrated the existence of P/Q-, N- and L-type VDCC, respectively. The remaining residual current could be blocked by cadmium. It was found that the contribution to the whole-cell current by the N-type channel was greater in TH+ cells than GAD+ cells at each time point examined, whilst the contribution to the whole-cell current by the L-type channel was greater in GAD+ cells than TH+ cells. However, over the 7-day culture period, the expression of VDCC types in both cell phenotypes changed in a similar fashion, with the contribution to the whole-cell current from the N-type current decreasing, and the contribution from the R-type current increasing. Our data could provide new insights into a range of neurodevelopmental mechanisms related to Ca(2+) homeostasis in developing mesencephalic neurons.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Mesencéfalo
/
Canais de Cálcio
/
Diferenciação Celular
/
Cálcio
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Sinalização do Cálcio
/
Neurônios
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2002
Tipo de documento:
Article