Search for new urinary biochemical markers of collagen degradation in man.
Clin Chim Acta
; 327(1-2): 81-6, 2003 Jan.
Article
em En
| MEDLINE
| ID: mdl-12482621
ABSTRACT
BACKGROUND:
In the human body, collagen degradation produces various fragments released from the extracellular matrix, which end up in the urine as pyridinium cross-links, either free or bound to peptides or sugars.METHODS:
We developed a high-performance liquid chromatography (HPLC) technique, which measures not only free pyridinoline (Pyr) and deoxypyridinoline (Dpyr), but also glucosyl-galactosyl-pyridinoline (glc-gal-pyr) and four still unidentified bound pyridinolines. Assuming that in Paget's disease of bone and in growing children the cross-links (CL) mainly originate from bone tissue, whereas after spinal cord injury their high excretion rather reflects the degradation of non-osseous collagen, we compared the urinary output of seven different cross-links, among which four had not been described before.RESULTS:
Our results show that one of those, which we called CL1, is essentially originating from bone and is more specific in this respect than Dpyr, whereas glc-gal-pyr also reflects the degradation of non-osseous collagen. The best indicator of non-bone collagen degradation was CL3, one of the newly discovered cross-links.CONCLUSIONS:
In conclusion, we demonstrate that new sensitive pyridinoline cross-links can be identified and assayed in human urine and that their specificity helps to distinguish diseases related to bone collagen from those in which other forms of collagen are involved.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biomarcadores
/
Colágeno
Tipo de estudo:
Diagnostic_studies
/
Observational_studies
/
Prognostic_studies
Limite:
Adolescent
/
Adult
/
Child
/
Child, preschool
/
Humans
/
Male
/
Middle aged
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article