Resumption of metabolic activity of vitrified/warmed ovine embryos.
Mol Reprod Dev
; 64(2): 207-13, 2003 Feb.
Article
em En
| MEDLINE
| ID: mdl-12506353
ABSTRACT
To evaluate resumption of metabolic activity of vitrified ovine embryos during a short time immediately after warming, blastocysts collected from superovulated Sarda ewes were incubated with (35)S-methionine. In vitrified/warmed embryo groups the protein secretion significantly (P < 0.05) increased from 0 to 24 hr of culture, reaching significantly (P < 0.01) higher activity at 18-24 hr and dropping to values similar to the control nonvitrified embryo group at 29-35 hr. Within the control group at 29-35 hr there was a significantly (P < 0.01) higher level of protein secretion compared to the other interval times. The electrophoretic pattern showed a 48-50 kDa secreted protein identified as urokinase-type plasminogen activator (PA). The caseinolytic assay of PA activity showed a course similar to protein secretion in both vitrified and control groups. During 29-35 hr of culture, we did not observe any improvement in PA activity as seen for secreted proteins. At this time, we observed the secretion of a new 20 kDa protein that was not present in vitrified/warmed embryos. Analysis of BrDU incorporation in newly synthesised DNA showed a significant (P < 0.01) improvement in positive cell number from 3 to 9 hr after warming, reaching a value similar to that of the control group at 12 hr of culture. Our results suggest that vitrified/warmed embryos require 9-12 hr of culture to complete resumption of DNA synthesis and 29-35 hr to re-acquire the full capacity of protein secretion but not the qualitative secretion pattern.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Blastocisto
/
Criopreservação
Tipo de estudo:
Qualitative_research
Limite:
Animals
/
Pregnancy
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article