Protein synthesis initiation factors from chicken erythroblasts.
Biochim Biophys Acta
; 425(2): 229-33, 1976 Mar 04.
Article
em En
| MEDLINE
| ID: mdl-1252502
ABSTRACT
Two factors (IF-I and IF-II) necessary for the initiation of protein synthesis have been partially purified from a 0.5 M KC1 wash of chicken erythroblast polysomes. IF-I mediates the binding of the initiator tRNA and GTP to a 40 S ribosomal subunit, resulting in the formation of a 44 S initiation intermediate. In the presence of IF-II and a suitable RNA template, the 44 S initiation intermediate combines with a 60 S ribosomal subunit to form a functional 80 S initiation complex. The methionyl moiety of the initiator tRNA in the 80 S initiation complex is able to react with puromycin to form methionylpuromycin.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Iniciação de Peptídeos
/
Eritrócitos
Limite:
Animals
Idioma:
En
Ano de publicação:
1976
Tipo de documento:
Article