Application of Agilent 2100 Bioanalyzer in detection of human papilloma virus.
Di Yi Jun Yi Da Xue Xue Bao
; 23(3): 213-5, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12651231
ABSTRACT
OBJECTIVE:
To explore the feasibility of using Agilent 2100 bioanalyzer in the detection and genotyping of human papilloma virus (HPV).METHODS:
The consensus sequence of highly conserved region (L1) and genotype-specific gene fragments of all HPV genotypes (including HPV6, 11, 16 and 18) were amplified by PCR technique using general and type-specific primers respectively. Agilent 2100 Bioanalyzer and routine agarose gel electrophoresis were then employed respectively to examine the PCR products, and the accuracy, reproducibility and sensitivity of these 2 detection techniques were compared.RESULTS:
Agilent 2100 Bioanalyzer showed better accuracy in determining the length of the gene fragments than agarose gel electrophoresis, the accuracy of the former reaching above 95% while the latter was only 85%. Bioanalyzer was also 100 times more sensitive than agarose electrophoresis.CONCLUSIONS:
Agilent 2100 Bioanalyzer combined with PCR is specific, accurate and sensitive for HPV detection and genotyping.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Papillomaviridae
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article