Differential expression of Ran GTPase during HMBA-induced differentiation in murine erythroleukemia cells.
Leuk Res
; 27(7): 607-15, 2003 Jul.
Article
em En
| MEDLINE
| ID: mdl-12681360
ABSTRACT
Murine erythroleukemia (MEL) cells undergo erythroid differentiation in vitro when treated with hexamethylene bisacetamide (HMBA). To identify genes involved in the commitment of MEL cells to differentiate, we screened a cDNA library constructed from HMBA-induced cells by differential hybridization and isolated GTPase Ran as a down-regulated gene. We observed that Ran was expressed in a biphasic mode. Following a decrease in mRNA level during the initial hours of induction, Ran re-expressed at 24-48 h, and gradually declined again. To investigate the role of Ran during MEL differentiation we constructed MEL transfectants capable to express or block Ran mRNA production constitutively. No effects were observed on cell growth and proliferation. Blockage of Ran, however, interfered with MEL cell differentiation resulting in a decrease of cell survival in the committed population.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Eritroblástica Aguda
/
Diferenciação Celular
/
Proteína ran de Ligação ao GTP
/
Acetamidas
/
Antineoplásicos
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article