The cyclopentenone 15-deoxy-delta 12,14-prostaglandin J2 binds to and activates H-Ras.
Proc Natl Acad Sci U S A
; 100(8): 4772-7, 2003 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-12684535
ABSTRACT
The cyclopentenone 15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)) induces cell proliferation and mitogen-activated protein kinase activation. Here, we describe that these effects are mediated by 15d-PGJ(2)-elicited H-Ras activation. We demonstrate that this pathway is specific for H-Ras through the formation of a covalent adduct of 15d-PGJ(2) with Cys-184 of H-Ras, but not with N-Ras or K-Ras. Mutation of C184 inhibited H-Ras modification and activation by 15d-PGJ(2), whereas serum-elicited stimulation was not affected. These results describe a mechanism for the activation of the Ras signaling pathway, which results from the chemical modification of H-Ras by formation of a covalent adduct with cyclopentenone prostaglandins.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Prostaglandina D2
/
Proteínas ras
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article