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Antibodies to Schistosoma mansoni in human cerebrospinal fluid.
Magalhães-Santos, Isis F; Lemaire, Denise C; Andrade-Filho, Antonio S; Queiroz, Aristides C; Carvalho, Otávio M; Carmo, Theomira M A; Siqueira, Isadora C; Andrade, Débora M; Rego, Michely F; Guedes, Ana Paula T; Reis, Mitermayer G.
Afiliação
  • Magalhães-Santos IF; Centro de Pesquisas Gonçalo Moniz-FIOCRUZ, Oswaldo Cruz Foundation, Salvador, Bahia, Brazil.
Am J Trop Med Hyg ; 68(3): 294-8, 2003 Mar.
Article em En | MEDLINE | ID: mdl-12685632
Cerebrospinal fluid (CSF) and serum samples from patients suspected of having neuroschistosomiasis (NS) were evaluated by an enzyme-linked immunosorbent assay. Monoclonal antibodies of various immunoglobulin isotypes (IgM, IgA, IgE, total IgG, IgG1, IgG2, IgG3, and IgG4) were used to detect antibodies against Schistosoma mansoni soluble egg antigen (SEA) and soluble worm adult preparation (SWAP). Of the 83 CSF samples tested, 55% were reactive to SEA (26% were reactive only to SEA and 29% to both SEA and SWAP), 34% were reactive to SWAP (5% only to SWAP and 29% to both SEA and SWAP), and 40% were not reactive with any antigen. Cases that tested positive for SWAP in CSF and negative in serum were not found. Samples with high specific IgG antibody titers were selected for immunoglobulin isotype profiling. In the CSF samples, the antibodies against SEA and SWAP were mainly IgM, IgG1, and IgG4, although other immunoglobulins were also detected. Interestingly, nine patients had high levels of IgG1 only in the CSF. These results suggest that there is local synthesis of IgG1, and that this isotype could be an important immunologic marker in the diagnosis of NS.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schistosoma mansoni / Esquistossomose / Anticorpos Anti-Helmínticos / Doenças do Sistema Nervoso Limite: Adolescent / Adult / Aged / Animals / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2003 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Schistosoma mansoni / Esquistossomose / Anticorpos Anti-Helmínticos / Doenças do Sistema Nervoso Limite: Adolescent / Adult / Aged / Animals / Child / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2003 Tipo de documento: Article