Initiation factor eIF2B not p70 S6 kinase is involved in the activation of the PI-3K signalling pathway induced by the v-src oncogene.
FEBS Lett
; 543(1-3): 81-6, 2003 May 22.
Article
em En
| MEDLINE
| ID: mdl-12753910
ABSTRACT
Our data show that in hamster fibroblasts transformed by Rous sarcoma virus (RSV), the phosphoinositide 3'-kinase (PI-3K)/Akt/glycogen synthase kinase 3 antiapoptotic pathway is upregulated and involved in increased protein synthesis through activation of initiation factor eIF2B. Upon inhibition of PI-3K by wortmannin, phosphorylation of 70-kDa ribosomal protein S6 kinase (p70 S6k) and its physiological substrate, ribosomal protein S6, decreased in the non-transformed cells but not in RSV-transformed cells. Thus PI-3K, which is thought to be involved in regulation of p70 S6k, signals to p70 S6k in normal fibroblasts, but it does not appear to be an upstream effector of p70 S6k in fibroblasts transformed by v-src oncogene, suggesting that changes in the PI-3K signalling pathway upstream of p70 S6k are induced by RSV transformation.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transdução de Sinais
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Proteína Oncogênica pp60(v-src)
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Proteínas Serina-Treonina Quinases
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Fosfatidilinositol 3-Quinases
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Fator de Iniciação 2B em Eucariotos
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Proteínas Quinases S6 Ribossômicas 70-kDa
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article