Xerocomus chrysenteron lectin: identification of a new pesticidal protein.
Biochim Biophys Acta
; 1621(3): 292-8, 2003 Jun 11.
Article
em En
| MEDLINE
| ID: mdl-12787928
ABSTRACT
Xerocomus chrysenteron is an edible mushroom with insecticidal properties. In an earlier work, we found that proteins are responsible for this toxicity. Here we describe the purification of a approximately 15 kDa lectin, named XCL, from the mushroom. Its cDNA and gDNA were cloned by PCR strategies and a recombinant form was expressed in Escherichia coli. Sequence alignments and sugar specificity showed that this protein is the third member of a new saline-soluble lectin family present in fungi. This protein, either purified from mushroom or expressed in vitro in E. coli, was found to be toxic to some insects, such as the dipteran Drosophila melanogaster and the hemipteran, Acyrthosiphon pisum. The lectin possesses a high insecticidal activity compared to lectin isolated from leguminosae (Lathyrus ochrus) or from the snowdrop (Galanthus nivalis).
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
/
Proteínas Fúngicas
/
Inseticidas
/
Lectinas
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article