Quantitation of anti-Pneumocystis jiroveci antibodies in healthy persons and immunocompromised patients.
J Infect Dis
; 187(12): 1844-8, 2003 Jun 15.
Article
em En
| MEDLINE
| ID: mdl-12792860
ABSTRACT
To facilitate studies of the epidemiology of Pneumocystis jiroveci infection in both healthy persons and immunocompromised patients, we developed a quantitative ELISA with recombinant major surface glycoprotein (MSG) fragment MSG-14, a P. jiroveci-specific protein that includes a highly conserved region of the MSG protein family. By immunoblot, all samples reacted with the carboxyl portion of MSG-14; by ELISA, immunocompromised patients with Pneumocystis pneumonia (PCP) who were immunocompromised for reasons other than AIDS had higher antibody levels than did either patients with AIDS with PCP (P=.01) or healthy persons (P=.005). Longitudinal observations of 8 patients with AIDS showed no correlation between time of diagnosis of Pneumocystis infection and change in antibody levels. Eleven percent (4/35) of healthy persons demonstrated a >4-fold change in antibody titers during 1 year of observation. This ELISA assay allows quantitation of anti-P. jiroveci antibodies in human serum samples and should be useful in better understanding the epidemiology of P. jiroveci infection in humans.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ascomicetos
/
Saúde
/
Hospedeiro Imunocomprometido
/
Anticorpos Antifúngicos
/
Micoses
Limite:
Adult
/
Female
/
Humans
/
Male
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article