Expression and radiation-induced phosphorylation of histone H2AX in mammalian cells.
J Radiat Res
; 44(1): 47-51, 2003 Mar.
Article
em En
| MEDLINE
| ID: mdl-12841599
ABSTRACT
The mouse histone H2AX (H2AX) has unique C-terminal Ser residues, which are phosphorylated in response to DNA double-strand breaks (DSBs) by ionizing radiation, suggesting that it plays a role in the maintenance of genomic stability. Here, we show that the H2AX protein was detected in most cells in various tissues, and was abundant in the S phase of the cell cycle. Following X-ray irradiation, H2AX was phosphorylated (gamma-H2AX) in the thymus, small intestine and testis. However, H2AX in epithelial cells in the villi of the small intestine were not strongly phosphorylated, even after X-irradiation. Thus, H2AX was expressed in almost all cells. However, the cells that expressed H2AX were not always phosphorylated by X-irradiation, suggesting a different mechanism of kination in those cells.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lesões por Radiação
/
Dano ao DNA
/
Histonas
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article