Fluorescence lifetime imaging for the two-photon microscope: time-domain and frequency-domain methods.
J Biomed Opt
; 8(3): 381-90, 2003 Jul.
Article
em En
| MEDLINE
| ID: mdl-12880343
ABSTRACT
Fluorescence lifetime images are obtained with the laser scanning microscope using two methods:
the time-correlated single-photon counting method and the frequency-domain method. In the same microscope system, we implement both methods. We perform a comparison of the performance of the two approaches in terms of signal-to-noise ratio (SNR) and the speed of data acquisition. While in our practical implementation the time-correlated single-photon counting technique provides a better SNR for low-intensity images, the frequency-domain method is faster and provides less distortion for bright samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Algoritmos
/
Aumento da Imagem
/
Adenilato Quinase
/
Neoplasias Laríngeas
/
Músculo Esquelético
/
Microscopia Confocal
/
Cadeias beta de Integrinas
/
Microscopia de Fluorescência por Excitação Multifotônica
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article