Low pH enhances Sp1 DNA binding activity and interaction with TBP.
Nucleic Acids Res
; 31(15): 4523-30, 2003 Aug 01.
Article
em En
| MEDLINE
| ID: mdl-12888513
ABSTRACT
Sp1 is involved in the regulation of a wide variety of genes, including housekeeping genes and genes involved in tumor growth. Sp1 is a member of the C2-H2 zinc-finger family and is important for protection against cellular acidosis in cells that grow under hypoxic or acidic conditions, such as tumor cells. To obtain an insight into the molecular mechanisms underlying pH-dependent transcription by Sp1, both its DNA binding activity and its interaction with TATA binding protein (TBP) were investigated under various pH conditions. We show here that the DNA binding activity of Sp1 increased and Sp1 formed a stable interaction with TBP at low pH. These findings indicate that pH changes significantly modulate the activity of Sp1 and thus contribute to the cellular response under hypoxic or acidic conditions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Fator de Transcrição Sp1
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Proteína de Ligação a TATA-Box
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article