Cultured rat growth plate chondrocytes express low levels of 1alpha-hydroxylase.
Recent Results Cancer Res
; 164: 147-9, 2003.
Article
em En
| MEDLINE
| ID: mdl-12899519
ABSTRACT
Growth plate chondrocytes are the target of the hydroxylated vitamin D metabolites 1alpha,25(OH)2D3 and 24,25(OH)2D3. Because studies on the production of these polar metabolites were inconclusive in various in vitro systems, the expression of a potential paracrine/autocrine vitamin D system was examined in primary cultures of rat growth plate chondrocytes using real-time RT-PCR. Compared to UMR cells and renal homogenates primary cultures of growth plate chondrocytes expressed low levels of 25-hydroxy-1alpha-hydroxylase as well as 25-hydroxy-24-hydroxylase. The expression of both is modulated by 25 vitamin D3, but 1alpha,25(OH)2D3 affected only 25-hydroxy-24-hydroxylase. If these findings are confirmed in intact growth plates, the polar vitamin D metabolites could act in a paracrine/autocrine fashion within the growth plate.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Esteroide Hidroxilases
/
Condrócitos
/
Sistema Enzimático do Citocromo P-450
/
Lâmina de Crescimento
/
25-Hidroxivitamina D3 1-alfa-Hidroxilase
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article