Detection of tomato yellow leaf curl virus by loop-mediated isothermal amplification reaction.
J Virol Methods
; 112(1-2): 35-40, 2003 Sep.
Article
em En
| MEDLINE
| ID: mdl-12951210
ABSTRACT
The genomic DNA molecule of tomato yellow leaf curl virus (TYLCV), a whitefly-transmitted geminivirus, was amplified from total DNA extracts of TYLCV-infected tomato (Lycopersicon esculentum) by the use of loop-mediated isothermal amplification (LAMP). The procedure was also used to amplify TYLCV DNA from total DNA extracts of individual whiteflies (Bemisia tabaci) that had fed on TYLCV-infected plants. One of the characteristics of the LAMP method is its ability to synthesize an extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophosphate ion, is produced yielding a white precipitate of magnesium pyrophosphate in the reaction mixture. The presence or absence of this white precipitate allows easy detection of amplification of TYLCV genomic DNA without gel electrophoresis.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Solanum lycopersicum
/
Geminiviridae
/
Técnicas de Amplificação de Ácido Nucleico
/
Hemípteros
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article