A group-specific, indirect sandwich ELISA for the detection of equine encephalosis virus antigen.
J Virol Methods
; 112(1-2): 129-35, 2003 Sep.
Article
em En
| MEDLINE
| ID: mdl-12951221
ABSTRACT
A polyclonal antibody-based, group-specific, indirect, sandwich ELISA (S-ELISA) for the detection of equine encephalosis virus (EEV) antigen was developed. Purified EEV particles were titrated in the S-ELISA and the limit of detection was determined to be approximately 9.0 ng of antigen/ml (0.45 ng/well). Positive S-ELISA reactions were recorded with seven serologically distinct EEV serotypes. No cross-reactions were recorded with other arboviruses including African horse sickness virus (AHSV) serotypes 1-9, bluetongue serotypes 1-24, epizootic haemorrhagic disease serotypes 1-8 and isolate 318, and selected isolates of Palyam, Eubenangee, Corriparta, Warrego, Akabane and bovine ephemeral fever viruses. The assay proved to be sensitive and specific for the rapid detection of EEV in cell cultures and in homogenated suckling mouse brain (MB). The data generated in this study suggest that the ELISA will be valuable for epidemiological studies of EE and will assist in making a reliable differential diagnosis between EEV and AHSV infections.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Reoviridae
/
Ensaio de Imunoadsorção Enzimática
/
Infecções por Reoviridae
/
Doenças dos Cavalos
/
Antígenos Virais
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article