[Determination of deoxyribonucleic acid with alkali blue 6B by resonance light-scattering method].
Guang Pu Xue Yu Guang Pu Fen Xi
; 23(3): 458-60, 2003 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-12953513
ABSTRACT
Characteristics of the interaction between alkali blue 6B with deoxyribonucleic acid (DNA) was studied by resonance light-scattering (RLS) method in the pH 2.90 medium. The effects of interference substances and the optimization of procedure were investigated, and a new method based on RLS was established. The method can be used to determine the DNA at nanogram level. The linear range of the DNA is 10-100.4 micrograms.L-1 with a correlation coefficient of 0.9913. The detection limit is 4.2 micrograms-L-1. The method has been applied to the determination of DNA in mixed samples with satisfactory results.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Benzenossulfonatos
/
DNA
/
Compostos de Anilina
Idioma:
Zh
Ano de publicação:
2003
Tipo de documento:
Article