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Interlaboratory ring test of time-resolved fluoroimmunoassays for zeranol and alpha-zearalenol and comparison with zeranol test kits.
Cooper, K M; Ribeiro, L; Alves, P; Vozikis, V; Tsitsamis, S; Alfredsson, G; Lövgren, T; Tuomola, M; Takalo, H; Iitia, A; Sterk, S S; Blokland, M; Kennedy, D G.
Afiliação
  • Cooper KM; Queen's University Belfast, Department of Veterinary Science, Belfast, UK.
Food Addit Contam ; 20(9): 804-12, 2003 Sep.
Article em En | MEDLINE | ID: mdl-13129775
Many zeranol immunoassay test kits cross-react with toxins formed by naturally occurring Fusarium spp. fungi, leading to false-positive screening results. This paper describes the evaluation and application of recently published, dry reagent time-resolved fluoroimmunoassays (TR-FIA) for zeranol and the toxin alpha-zearalenol. A ring test of bovine urine fortified with zeranol and/or alpha-zearalenol in four European Union National Reference Laboratories demonstrated that the TR-FIA tests were accurate and robust. The alpha-zearalenol TR-FIA satisfactorily quantified alpha-zearalenol in urine fortified at 10-30 ng ml(-1). The specificity-enhanced zeranol TR-FIA accurately quantified zeranol in the range 2-5 ng ml(-1) and gave no false-positive results in blank urine, even in the presence of 30 ng ml(-1) alpha-zearalenol. Zeranol TR-FIA specificity was demonstrated further by analysing incurred zeranol-free urine samples containing natural Fusarium spp. toxins. The TR-FIA yielded no false-positive results in the presence of up to 22 ng ml(-1) toxins. The performance of four commercially available zeranol immunoassay test kits was more variable. Three kits produced many false-positive results. One kit produced only one potential false-positive using a protocol that was longer than that of the TR-FIA. These TR-FIAs will be valuable tools to develop inspection criteria to distinguish illegal zeranol abuse from contamination arising from in vivo metabolism of Fusarium spp. toxins.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zeranol / Bovinos / Detecção do Abuso de Substâncias / Estrogênios não Esteroides Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Ano de publicação: 2003 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zeranol / Bovinos / Detecção do Abuso de Substâncias / Estrogênios não Esteroides Tipo de estudo: Evaluation_studies Limite: Animals Idioma: En Ano de publicação: 2003 Tipo de documento: Article