Block of Ca2+ wave and Ca2+ oscillation by antibody to the inositol 1,4,5-trisphosphate receptor in fertilized hamster eggs.
Science
; 257(5067): 251-5, 1992 Jul 10.
Article
em En
| MEDLINE
| ID: mdl-1321497
ABSTRACT
The concentration of cytoplasmic free calcium (Ca2+) increases in various stimulated cells in a wave (Ca2+ wave) and in periodic transients (Ca2+ oscillations). These phenomena are explained by inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release (IICR) and Ca(2+)-induced Ca2+ release (CICR) from separate intracellular stores, but decisive evidence is lacking. A monoclonal antibody to the IP3 receptor inhibited both IICR and CICR upon injection of IP3 and Ca2+ into hamster eggs, respectively. The antibody completely blocked sperm-induced Ca2+ waves and Ca2+ oscillations. The results indicate that Ca2+ release in fertilized hamster eggs is mediated solely by the IP3 receptor, and Ca(2+)-sensitized IICR, but not CICR, generates Ca2+ waves and Ca2+ oscillations.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Óvulo
/
Canais de Cálcio
/
Cálcio
/
Receptores Citoplasmáticos e Nucleares
/
Receptores de Superfície Celular
/
Fertilização
Limite:
Animals
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article