Identification of transglutaminase substrates in HT29 colon cancer cells: use of 5-(biotinamido)pentylamine as a transglutaminase-specific probe.
Biochim Biophys Acta
; 1136(1): 12-6, 1992 Jul 22.
Article
em En
| MEDLINE
| ID: mdl-1353685
ABSTRACT
A biotinamine probe, 5-(biotinamido)pentylamine, was used for biotin-labeling of proteins in HT29 colon cancer cell extracts by endogenous transglutaminase activity. The biotin-labeled protein substrates were isolated and recovered by avidin-affinity chromatography. The proteins were separated using SDS-polyacrylamide gel electrophoresis, electroblotted onto a polyvinylidene difluoride membrane, visualized using Coomassie blue, cut out, and sequenced. Amino acid sequence data identified human fructose-1,6-bisphosphate aldolase A, an intracellular protein, as a substrate for cellular transglutaminase.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sondas Moleculares
/
Transglutaminases
/
Frutose-Bifosfato Aldolase
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article