Stabilisation of eudesmane cation by tryptophan 334 during aristolochene synthase catalysis.

Deligeorgopoulou, Athina; Taylor, Susan E; Forcat, Silvia; Allemann, Rudolf K

Deligeorgopoulou, Athina; Taylor, Susan E; Forcat, Silvia; Allemann, Rudolf K.

Afiliação

Deligeorgopoulou A; School of Chemical Sciences, University of Birmingham, Edgbaston, Birmingham, UK B15 2TT.

Chem Commun (Camb) ; (17): 2162-3, 2003 Sep 07.

Article em En | MEDLINE | ID: mdl-13678181

RESUMO

Analysis of the hydrocarbons produced during catalysis by mutants of aristolochene synthase from Penicillium roqueforti indicated that Trp 334 had a pivotal function for the efficient production of aristolochene from farnesylpyrophosphate most likely by stabilising the intermediate, eudesmane cation.

Assuntos

Isomerases/metabolismo; Sesquiterpenos de Eudesmano; Sesquiterpenos/química; Triptofano/química; Catálise; Cátions; Penicillium/enzimologia; Penicillium/genética; Fosfatos de Poli-Isoprenil/química

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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sesquiterpenos / Triptofano / Sesquiterpenos de Eudesmano / Isomerases Idioma: En Ano de publicação: 2003 Tipo de documento: Article

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