The effect of superhelical density on the yield of single-strand breaks in gamma-irradiated plasmid DNA.
Radiat Res
; 132(1): 69-73, 1992 Oct.
Article
em En
| MEDLINE
| ID: mdl-1410276
ABSTRACT
Using agarose gel electrophoresis, the formation of DNA single-strand breaks (SSBs) by 137Cs gamma irradiation was quantified in negatively supercoiled topological isomers of plasmid pUC18. The G value for SSB formation falls slightly from 1 x 10(8) to 8 x 10(-9) SSB Gy-1 Da-1 as the superhelical density varies from 0.00 to -0.08. This result is not in agreement with recent observations by others which suggest that increasing the negative superhelical density of plasmid DNA increases its sensitivity to X irradiation.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmídeos
/
Dano ao DNA
/
DNA Bacteriano
/
DNA de Cadeia Simples
/
DNA Super-Helicoidal
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article