Hatching of common carp (Cyprinus carpio L.) embryos stored at 4 and -2 degrees C in different concentrations of methanol and sucrose.
Theriogenology
; 60(8): 1409-22, 2003 Nov.
Article
em En
| MEDLINE
| ID: mdl-14519463
ABSTRACT
The hatching performance of common carp (Cyprinus carpio L.) embryos was examined after 12-72-h storage at 4 and -2 degrees C using different concentrations of sucrose (0.1, 0.25, 0.5 and 1.0 M or 3.42, 8.55, 17.10 and 34.2%), methanol (MeOH) (0.5, 1.0, 1.5, 2.0, 2.5, 3.0 and 3.5 M or 1.6, 3.2, 4.8, 6.4, 8.0, 9.6 and 11.2%), or varying concentrations of methanol in 0.5 M (17.10%) sucrose. For sucrose, 0.5 M (17.10%) showed the maximum survival (41+/-1% (12 h) to 11+/-1.5% (72 h)) at 4 degrees C. No survival was observed at -2 degrees C with any concentration of sucrose. At both temperatures employed, hatching was higher with mixed combination of methanol (1.5 M or 4.8%) and 0.5 M (17.10%) sucrose (4 degrees C 41+/-1.5% (12 h), 38+/-1.2% (72 h); -2 degrees C 33+/-1.7% (12 h), 28+/-1.2% (72 h)) compared to methanol alone (4 degrees C 38+/-1.5% (12 h), 35+/-2.5% (72 h); -2 degrees C 31+/-2.5% (12 h), 25+/-2% (72 h)). The combination of 1.5 M (4.8%) methanol and 0.5 M (17.10%) sucrose produced the best results among all the concentrations tested at both temperatures.
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Base de dados:
MEDLINE
Assunto principal:
Sacarose
/
Preservação de Tecido
/
Carpas
/
Criopreservação
/
Crioprotetores
/
Metanol
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article