Alternative trans-splicing of constant and variable exons of a Drosophila axon guidance gene, lola.
Genes Dev
; 17(20): 2496-501, 2003 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-14522953
ABSTRACT
longitudinals lacking (lola) is a complex Drosophila gene encoding at least 20 protein isoforms,each bearing the same N-terminal constant region linked to a different C-terminal variable region. Different isoforms specify different aspects of axon growth and guidance. We show here that lola mRNAs are generated by alternative trans-splicing of exons sequentially encoded by the same DNA strand. Chromosomal pairing facilitates interallelic trans-splicing,allowing complementation between mutations in the constant and those in the variable exons. We demonstrate that at least one variable exon is transcribed from its own promoter,and trans-spliced to the constant exons transcribed separately.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Processamento Alternativo
/
Proteínas de Drosophila
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Drosophila
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article