Interfering with different steps of protein synthesis explored by transcriptional profiling of Escherichia coli K-12.
J Bacteriol
; 185(20): 6158-70, 2003 Oct.
Article
em En
| MEDLINE
| ID: mdl-14526028
ABSTRACT
Escherichia coli responses to four inhibitors that interfere with translation were monitored at the transcriptional level. A DNA microarray method provided a comprehensive view of changes in mRNA levels after exposure to these agents. Real-time reverse transcriptase PCRanalysis served to verify observations made with microarrays, and a chromosomal grpElux operon fusion was employed to specifically monitor the heat shock response. 4-Azaleucine, a competitive inhibitor of leucyl-tRNA synthetase, surprisingly triggered the heat shock response. Administration of mupirocin, an inhibitor of isoleucyl-tRNA synthetase activity, resulted in changes reminiscent of the stringent response. Treatment with kasugamycin and puromycin (targeting ribosomal subunit association as well as its peptidyl-transferase activity) caused accumulation of mRNAs from ribosomal protein operons. Abundant biosynthetic transcripts were often significantly diminished after treatment with any of these agents. Exposure of a relA strain to mupirocin resulted in accumulation of ribosomal protein operon transcripts. However, the relA strain's response to the other inhibitors was quite similar to that of the wild-type strain.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Transcrição Gênica
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Biossíntese de Proteínas
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Inibidores da Síntese de Proteínas
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Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
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Proteínas de Escherichia coli
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Aminoglicosídeos
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Leucina
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article