Expression and production of bioactive human interleukin-18 in transgenic tobacco plants.
Biotechnol Lett
; 25(19): 1629-35, 2003 Oct.
Article
em En
| MEDLINE
| ID: mdl-14584919
ABSTRACT
The cDNA of human interleukin-18 (hIL-18) was successfully inserted into the genome of tobacco plant, Nicotiana tabacum cv. NC-89, using Agrobacterium tumefaciens-mediated transformation. Insertion and translation of hIL-18 in transformants were confirmed by PCR, ELISA, and Western blot, respectively. The transformed extracts contained the recombinant hIL-18 protein up to 0.05% of total soluble protein. Activity of the recombinant hIL-18 in plant cells was confirmed by the induction of IFN-gamma on IL-18-responsive J6-1 cells by the extracts obtained from the transformants. The expression level of hIL-18 (351 ng g(-1) tobacco tissue) obtained in the present study may be sufficient to induce responses/effects in vivo.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Nicotiana
/
Engenharia de Proteínas
/
Plantas Geneticamente Modificadas
/
Regulação da Expressão Gênica de Plantas
/
Interleucina-18
Limite:
Humans
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article