Presence of methylated adenine in GATC sequences in chromosomal DNAs from Campylobacter species.
J Bacteriol
; 174(24): 8156-7, 1992 Dec.
Article
em En
| MEDLINE
| ID: mdl-1459965
ABSTRACT
We digested chromosomal DNAs from 12 Campylobacter strains (C. jejuni, 4 strains; C. coli, 2 strains; C. fetus subsp. fetus, 2 strains; C. hyointestinalis, 2 strains; and C. upsaliensis, 2 strains) and from 4 Helicobacter strains (H. pylori, 2 strains; and H. mustelae, 2 strains) with HindIII, SstI, BamHI, DpnI, MboI, and Sau3AI. Restriction fragments were then separated by electrophoresis in 1% agarose or 10% polyacrylamide gels. Only DNAs from three Campylobacter species (C. jejuni, C. coli, and C. upsaliensis) were digested with DpnI (an enzyme that recognizes only methylated adenine in GATC sequences). We used MboI and Sau3AI to confirm these findings.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Campylobacter
/
DNA Bacteriano
/
Adenina
/
Cromossomos Bacterianos
Idioma:
En
Ano de publicação:
1992
Tipo de documento:
Article