Two different clean-up procedures for liquid chromatographic determination of ochratoxin A in urine.
J Chromatogr B Analyt Technol Biomed Life Sci
; 798(2): 317-21, 2003 Dec 25.
Article
em En
| MEDLINE
| ID: mdl-14643512
ABSTRACT
This paper describes two different procedures for extraction of ochratoxin A (OTA) from urine samples one using acidic chloroform-methanol mixture, followed by solid-phase extraction (SPE) clean-up and the other using commercial Chem Elut columns and a chloroform-formic acid mixture. The recovery of OTA using the procedure with silica gel columns was 82% with a R.S.D. < 8.4% and the detection and quantitation limits were 0.5 and 1.5 ng OTA/ml, respectively. The recovery of OTA in the second procedure with urine samples purified only on commercial Chem Elut columns was 95% with R.S.D. < 4.0%, and detection and quantitation limits 0.3 and 0.9 ng/ml, respectively. Both procedures of OTA extraction effectively eliminate interfering substances and give reliable and repeatable results. However, the procedure with Chem Elut columns gave higher recovery and lower detection and quantitation limits. It was successfully applied in determining OTA in human urine samples.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Ocratoxinas
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article