Herbimycin A induces sympathetic neuron survival and protects against hypoxia.
Neuroreport
; 14(18): 2397-401, 2003 Dec 19.
Article
em En
| MEDLINE
| ID: mdl-14663199
ABSTRACT
We have examined how herbimycin affects the survival and neuritogenesis of avian sympathetic neurons. Herbimycin promoted sympathetic neuron survival and neuritogenesis. At higher concentrations (> or = 100 ng/ml), herbimycin still enhanced neuron survival but blocked neuritogenesis. Addition of herbimycin (10-30 ng/ml) to neurons cultured in the presence of NGF or retinal conditioned medium altered neuronal morphology, with an increase in the number of neurites. Addition of NGF during hypoxia rescued 52% of the neurons compared to 14% survival in control conditions. Herbimycin alone rescued about 50% of the neurons. In the presence of NGF and 100 ng/ml herbimycin, 81% of the neurons survived hypoxia. Our results show that herbimycin promotes survival of chick sympathetic neurons and potentiates the effects of NGF.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Quinonas
/
Fibras Adrenérgicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2003
Tipo de documento:
Article