Chromosome-protein interactions in polyomavirus virions.
J Virol
; 78(1): 513-9, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-14671132
ABSTRACT
In this work, we sought to determine whether the components of the murine polyomavirus capsid establish specific interactions with the minichromosome encapsidated into the mature viral particles by using the cis-diamminedichloroplatinum(II) cross-linking reagent. Our data indicated that VP1, but not minor capsid proteins, interacts with the viral genome in vivo. In addition, semiquantitative PCR assays performed on cross-linked DNA complexes revealed that VP1 binds to all regions of the viral genome but significantly more to the regulatory region. The implications of such an interaction for viral infectivity are discussed.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Vírion
/
Cromossomos
/
Polyomavirus
/
Proteínas do Capsídeo
Limite:
Animals
Idioma:
En
Ano de publicação:
2004
Tipo de documento:
Article